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Will the neural tissues divide to form more tissues ?

Neural tissues can be dissociated to single-cell suspensions by
enzymatic degradation of the extracellular adhesion proteins which
maintain the structural integrity of tissues.
The neural tissue can be dissociated either using the gentleMACS.
Dissociator or manually using Pasteur pipettes. After the tissue has
been cut into small pieces, a pre-warmed enzyme mix is added to
the tissue pieces and incubated with agitation at 37 °C. The tissue
is mechanically dissociated and the suspension is applied to a cell
strainer. Optionally, myelin can be removed using Myelin Removal
Beads II, as it can interfere with subsequent flow cytometric
analysis or cell separation using MACS Technology. Cells should
be processed immediately for downstream applications, such as
cellular or molecular analyses or cell separations.


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