Ask a Teacher



sir,can you please explain the technique of chromatography?also describe how can we separate coloured components of an ink?

Chromatography is the technique used for the separation and identification of substances present in complex mixtures .It involves the separation of different dissolved constituents of a mixture based on the difference in adsorption of the different constituents on the surface of an appropriate adsorbent material or solid medium .

Paper chromatography is used for the separation of coloring material in ink .
Step 1  :  A spot of ink is placed with the help of a capillary tube in the centre of a base line on the strip of filter paper .
Step 2   :  The spot is dried and and the paper is hung in a glass jar with its lower end immersed in the solvent .(75%-distilled water,15% -methylated spirit and 10%- soln of ammonium sulphate )
Step 3   :  The solvent flows over the spot and carries the components to distances along the paper indicated by colored spots .Colored spots are characteristic of each constituent in the mixture .


comments powered by Disqus