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sir,can you please explain the technique of chromatography?also describe how can we separate coloured components of an ink? |
Chromatography is the technique used for the separation and identification of substances present in complex mixtures .It involves the separation of different dissolved constituents of a mixture based on the difference in adsorption of the different constituents on the surface of an appropriate adsorbent material or solid medium . Paper chromatography is used for the separation of coloring material in ink . Step 1 : A spot of ink is placed with the help of a capillary tube in the centre of a base line on the strip of filter paper . Step 2 : The spot is dried and and the paper is hung in a glass jar with its lower end immersed in the solvent .(75%-distilled water,15% -methylated spirit and 10%- soln of ammonium sulphate ) Step 3 : The solvent flows over the spot and carries the components to distances along the paper indicated by colored spots .Colored spots are characteristic of each constituent in the mixture . |