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what is the correct sequence of the steps to observe the slide of amoeba under the microscope? a)adjust the diaphragm and the mirror of the microscope so that sufficient light may enter to illuminate the slide. b)fix the slide on the stage carefully. c)adjust the microscope to high power and focus. d)adjust the microscope to low power and focus.
Simple microscope:

Fix the slide on the stage carefully.
Adjust the diaphragm and the mirror of the microscope for sufficient light.
Adjust the microscope to low power and focus
Adjust the microscope to high power and focus.


Compound light microscope:

Place the slide on a white piece of paper find the specimen
Step
 Lock the low power objective lens into place.
   Start with either the low power or scanning objective when you want to view a slide.
 Use the coarse focus knob to move the stage (or objective lens) so that they are as far apart from  
each other as possible. Open the stage clip and place the slide snugly in the corner of the stage  
clip (make sure the slide is completely flat) before releasing the clip to hold the slide firmly in  
place. Then use the mechanical stage knobs to position the slide so that the specimen (i.e., letter  
“e”) is centered over the condenser and the light that passes through it.
Next, using the coarse focus knob once again, move the slide and objective lens as close together  
as the knob will allow.

 Now, look into the ocular lens(es). Using the coarse focus knob, SLOWLY increase the distance  
between the slide and objective until the specimen is in focus.  
If the light is too intense, adjust the diaphragm lever (or dial near the lamp if present)
until the light level is comfortable before trying to locate the specimen.
If you have difficulty locating and focusing on your specimen, make sure that it is properly centered and you may need to adjust the course focus more slowly.
 Adjust the diaphragm lever so there is sufficient contrast between the specimen and the  
background, closing it no more than is necessary. This step is especially important for live
specimens since you may not be able to see them otherwise.
 Now use the fine focus knob to get the specimen in proper focus. You should now be able to see  
the object clearly. Before going to the next step (increasing the magnification), be sure to center  
your specimen in the field of view as best you can.
. Now that you have centered and focused the object as best you can at low power, rotate the high power.


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