Which of the following shows correct chronological order of the events occurring during callus culture?
Callus→ Cell division→ Explant→ Addition of cytokinin→ Cells acquire meristematic property
Explant→ Callus→ Cell division→ Addition of cytokinin→ Cells acquire meristematic property
Explant→ Cell division→ Callus→ Addition of cytokinin→ Cells acquire meristematic property
Callus→ Explant→ Cell division→ Addition of cytokinin→ Cells acquire meristematic property
Continuous addition of sugars in 'fed batch' fermentation is done to
obtain antibiotics
purify enzymes
degrade sewage
produce methane
The process , in which mature differentiated cells reverse to meristematic activity to form callus is called
de-differentiation
differentiation
cyto-differentiation
re-differentiation
In crop improvement programmes,virus-free clones can be obtained through
grafting
hybridization
embryo culture
shoot apex culture
In order to obtain virus-free plants through tissue culture, the best method is
protoplast culture
embryo rescue
anther culture
meristem culture
In the initial stages of protoplast culture,sorbitol/mannitol is added
as an additional source of carbon
as an additional source of energy
to keep cells alive after the removal of cell wall
as an osmotic stabilizer
Which one of the following is the most suitable,medium for culture of Drosophila melanogaster?
Moist bread
Agar agar
Ripe banana
Cow dung
In tissue culture,roots can be induced by
lower concentration of cytokinin and higher concentration of auxins.
only cytokinin and no auxins
no cytokinin and only auxins
higher concentration of cytokinin and lower concentration of auxins
Hardening in tissue culture is
keeping at 30-50ºC temperature for about 30 minutes.
acclimatization tissue culture plants slowly before growing in the field.
plunging the vials into water at 37-40ºC
none of the above
A nutritionally wild type organism, which does not require any additional growth supplement is known as
phenotype
holotype
auxotroph
prototroph
